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<p class="ql-align-justify">Immunohistochemical studies were performed with appropriate positive and negative controls.</p><p><br></p><ul><li><strong>Synaptophysin</strong>: Diffuse cytoplasmic positivity in tumor cells.</li><li><strong>CD99</strong>: Diffuse, strong membranous positivity.</li><li><strong>LCA (CD45):</strong> Negative in tumor cells.</li><li><strong>Cytokeratin (pan-CK):</strong> Negative in tumor cells.</li><li><strong>Desmin</strong>: Negative in tumor cells.</li><li><strong>Myogenin</strong>: Negative in tumor cells.</li><li><strong>SOX10</strong>: Negative in tumor cells.</li><li class="ql-align-justify"><strong>Ki-67 proliferation index:</strong> Approximately 50%, assessed in tumor cell hot spots.</li></ul>
<p>Referred paraffin block of biopsy from left perianal mass.</p>
<p class="ql-align-justify">One referred paraffin block of previously submitted biopsy coded 878-2026.</p>
<p><strong><em><u>Left perianal mass, biopsy and immunohistochemistry:</u></em></strong></p><ul><li><strong>Consistent with extraskeletal Ewing sarcoma / primitive neuroectodermal tumor (PNET).</strong></li></ul><p><br></p><p><strong><u>Comment</u></strong><u>:</u></p><h5 class="ql-align-justify">For more confirmation additional marker study (NKX2.2 and molecular study for EWSR1 gene rearrangement is recommended.</h5>
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